15th - 20th September 2009
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Conference Secretariat
Via R. Guardini 8 - 38100 Trento Italy
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info@eaaitaly2009.com
Scientific Secretariat
scientific_s@eaaitaly2009.com
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> Gender and mitochondrial haplogroup identification of infant remains revealed in Tell Yunacite by molecular genetic methods
Molecular genetic methods are implemented for gender identification of remains if traditional anthropology methods are not applicable for some reason. Molecular genetic approaches to human gender identification are based on isolation of genomic DNA from available biological materials and subsequent PCR amplification of loci located on sex chromosomes (X and Y). An approach to identifying mitochondrial haplogroups is based on the detection of! diagnostic mutations in the hypervariable region of the mitochondrial DNA. 28 infant burials have been registered in the Early Bronze Age cultural deposits of Tell Yunacite (Thracian Plain, Bulgaria). 26 of them were discovered within the dwellings, the remaining two were located near the houses. All infants belonged to the age group under 1.8 years old which makes anthropological methods not applicable for gender identification. At the current stage of the research bone samples from 10 burials have been analyzed using the molecular biology methods. Unambiguous results of gender identification for six samples were obtained. Data acquired using the applied molecular assays reveal that all six samples belong to male infants. Mitochondrial DNA study is the second goal. The results of the detection of the diagnostic mutations in three infant DNA samples show that two individuals belong to the HV0 haplogroup and the third individual possesses genetic markers for both HV0 and th! e related H6 haplogroups. These haplogroups are rather typical! for mod ern Europeans. Our study could provide data crucial for the comprehension of historical and cultural features of ancient inhabitants of the region.
e-mail: kuralexus@rambler.ru
GENDER AND MITOCHONDRIAL HAPLOGROUP IDENTIFICATION OF INFANT REMAINS REVEALED IN TELL YUNACITE BY MOLECULAR GENETIC METHODS
Alexey KurnosovMolecular genetic methods are implemented for gender identification of remains if traditional anthropology methods are not applicable for some reason. Molecular genetic approaches to human gender identification are based on isolation of genomic DNA from available biological materials and subsequent PCR amplification of loci located on sex chromosomes (X and Y). An approach to identifying mitochondrial haplogroups is based on the detection of! diagnostic mutations in the hypervariable region of the mitochondrial DNA. 28 infant burials have been registered in the Early Bronze Age cultural deposits of Tell Yunacite (Thracian Plain, Bulgaria). 26 of them were discovered within the dwellings, the remaining two were located near the houses. All infants belonged to the age group under 1.8 years old which makes anthropological methods not applicable for gender identification. At the current stage of the research bone samples from 10 burials have been analyzed using the molecular biology methods. Unambiguous results of gender identification for six samples were obtained. Data acquired using the applied molecular assays reveal that all six samples belong to male infants. Mitochondrial DNA study is the second goal. The results of the detection of the diagnostic mutations in three infant DNA samples show that two individuals belong to the HV0 haplogroup and the third individual possesses genetic markers for both HV0 and th! e related H6 haplogroups. These haplogroups are rather typical! for mod ern Europeans. Our study could provide data crucial for the comprehension of historical and cultural features of ancient inhabitants of the region.
e-mail: kuralexus@rambler.ru
